84-5324-49　UBB 200ul　529432

特徴

• Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway.
• Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome.
• Three components are involved in the target protein-ubiquitin conjugation process.
• Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3).
• The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities.
• Several proteins such as IkappaB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin- proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.