The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations.
Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1).
In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2).
While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2).
Amplification of the p63 gene is frequently observed in squamous cell carcinomas of the lung, head and neck (2,3).
The p63 gene contains an alternative transcription intiation site that yields a 40kD deltaNp63 lacking the transactivation domain, and alternative splicing at the C-terminus yields the alpha, beta and gamma isoforms (3,4).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human TP63. Species Crossreactivity: Mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to human TP63.
