84-5319-59　TNFSF13 100ul　529179

特徴

• BAFF, a member of the TNF superfamily of proteins, is a homotrimeric transmembrane protein, which is cleaved to produce a soluble cytokine (1).
• BAFF may also further oligomerize into 60-mer structures (1).
• BAFF is expressed by neutrophils, macrophages, dendritic cells, activated T cells, and epithelial cells (1,2).
• BAFF plays a key role in B cell development, survival, and activation (1,3,4).
• BAFF binds to three distinct receptors, BAFF-R, TACI, and BCMA (1).
• These receptors are differentially expressed during B cell development and among B cell subsets (1,2,4).
• While BAFF-R and BCMA bind to the homotrimeric form of BAFF, TACI only binds to membrane bound or higher order BAFF structures (1).
• The BAFF/ BAFF-R interaction activates both canonical and non-canonical NF-κB pathways, PI3K/Akt, and mTOR (2,4).
• Activation of the noncanonical NF-κB pathway via BAFF-R is negatively regulated by TRAF3 (5).
• Elevated levels of BAFF may exacerbate many autoimmune disorders, making it an attractive therapeutic target (2).
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.