84-5316-79　TGFA 200ul　529033

特徴

• Transforming growth factor alpha (TGF-alpha) is a member of the epidermal growth factor (EGF) family, sharing the same receptor, EGFR, and regulating cell proliferation, survival, and differention (1).
• Members of the family share an EGF-like domain of aa45-60 characterized by the conservation of six regularly spaced cysteins, forming three disulfide bonds that function as their receptor binding domain.
• TGF-alpha was initially discovered in the media of retrovirally transformed fibroblasts, and it name comes from its ability to induce transformation in cultured fibroblasts (2).
• This transforming activity was later shown to require TGF- beta, which potentiates the activity of TGF-alpha through a separate receptor (3).
• Soluble TGF-alpha is released from its membrane-bound precusor, pro-TGF-alpha, following protolytic cleavage, but the membrane bound precursor is still able to bind and activate EGFR (4).
• Binding of soluble or membrane bound TGF-alpha to EGFR leads to receptor dimerization, tyrosine autophosphorylation, and activation of downstream signaling components.
• TGF-alpha and related peptides play an important role in the progression of cancer as well as in neuropathological processes (5,6).
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.