84-5315-33　TCEB2 100ul　528957

特徴

• This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex.
• The SIII complex is composed of elongins A/A2, B and C.
• It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units.
• Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits.
• Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C.
• The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.
• Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Pseudogenes have been identified on chromosomes 11 and 13.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.