84-5314-75　TBP 200ul　528927

特徴

• The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box.
• This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters.
• TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex.
• As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene.
• However, it is estimated that only 10-20% of human promoters have TATA boxes.
• Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II.
• This protein is not suitable for samples where the nuclear envelope has been removed.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.