84-5310-42　STMN1 100ul　528705

特徴

• Stathmin is a ubiquitously expressed microtubule destabilizing phosphoprotein that is upregulated in a number of cancers.
• The N-terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization.
• Phosphorylation at these sites inactivates the protein and stabilizes microtubules.
• Ser16 phosphorylation by CaM kinases II and IV (1,2) increases during G2/M-phase and is involved in mitotic spindle regulation (3,4).
• Ser38 is a target for cdc2 kinase (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6).
• EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7).
• Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3' and RB3''.
• Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC12 cells (8).
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.