84-5309-97　STEA3 100ul　528682

特徴

• STEAP3 a multi-pass membrane protein induced directly by p53.
• Plays an important role in facilitating exosome secretion.
• Enhances secretion in the DNA damage-induced p53-dependent exosomal secretory pathway.
• Localizes to vesicular-like structures at the plasma membrane and around the nucleus.
• Highly expressed in hematopoietic tissues where it localizes to the specialized endosome.
• An endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells.
• Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+).
• Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis.
• Uses NAD(+) as acceptor.
• Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.