84-5306-25　SNAI2 200ul　528476

特徴

• Slug (SNAI2) is a widely expressed transcriptional repressor and member of the Snail family of zinc finger transcription factors (1).
• Similar to the related Snail protein, Slug binds to the E-cadherin promoter region to repress transcription during development (2).
• The binding of Slug to integrin promoter sequences represses integrin expression and results in reduced cell adhesion (3).
• Down regulation of E- cadherin expression occurs during the epithelial- mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (4,5).
• The tumor suppressor protein p53 induces Slug expression in γ- irradiated cells; Slug protects damaged cells from apoptosis by repressing p53-induced transcription of the proapoptotic Bcl-2 family protein Puma (6).
• Deletion mutations in the corresponding Slug gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism, while a genetic duplication resulting in Slug overexpression is associated with a collection of congenital heart defects termed tetralogy of Fallot (7).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.