84-5302-60　SIX3 100ul　528286

特徴

• Transcriptional regulator which can act as both a transcriptional repressor and activator by binding a ATTA homeodomain core recognition sequence on these target genes.
• During forebrain development represses WNT1 expression allowing zona limitans intrathalamica formation and thereby ensuring proper anterio-posterior patterning of the diencephalon and formation of the rostral diencephalon.
• Acts as a direct upstream activator of SHH expression in the rostral diencephalon ventral midline and that in turn SHH maintains its expression.
• In addition, Six3 activity is required for the formation of the telencephalon.
• During postnatal stages of brain development is necessary for ependymal cell maturation by promoting the maturation of radial glia into ependymal cells through regulation of neuroblast proliferation and migration.
• Acts on the proliferation and differentiation of neural progenitor cells through activating transcription of CCND1 AND CCND2.
• During early lens formation plays a role in lens induction and specification by activating directly PAX6 in the presumptive lens ectoderm.
• In turn PAX6 activates SIX3 resulting in activation of PDGFRA and CCND1 promoting cell proliferation.
• Also is required for the neuroretina development by directly suppressing WNT8B expression in the anterior neural plate territory.
• Its action during retina development and lens morphogenesis is AES and TLE4-dependent manner.
• Furthermore, during eye development regulates several genes expression.
• Before and during early lens development represses the CRYGF promoter by binding a SIX repressor element.
• Directly activates RHO transcription, or cooperates with CRX or NRL.
• Six3 functions also in the formation of the proximodistal axis of the optic cup, and promotes the formation of optic vesicles-like structures.
• During pituitary development, acts in parallel or alternatively with HESX1 to control cell proliferation through Wnt/beta-catenin pathway (By similarity).
• Plays a role in eye development by suppressing WNT1 expression and in dorsal-ventral patterning by repressing BMP signaling pathway.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.