84-5302-21　Sin1, phosphorylated (Thr86) 200ul　528265

特徴

• Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals.
• mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive.
• mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors.
• mTORC2 promotes the serum-induced formation of stress-fibers or F-actin.
• mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation.
• mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'.
• mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
• Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity.
• MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation.
• Inhibits HRAS and KRAS signaling.
• Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.
• Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.