84-5299-93　SERPINF1 100ul　528150

特徴

• Pigment epithelium-derived growth factor (PEDF), also known as EPC-1 (early population doubling level cDNA-1), is a glycoprotein found naturally in the normal eye.
• PEDF has reported neuroprotective and differentiation properties and is secreted in abundance by retinal pigment epithelium cells.
• It belongs to the serine protease inhibitor (Serpin) superfamily and has been reported to inhibit angiogenesis and proliferation of several cell types.
• The “pooling” of PEDF within the interphotoreceptor matrix places this molecule in a prime physical location to affect the underlying neural retina.
• Additionally, PEDF induces neuronal differentiation and promotes survival of neurons of the central nervous system from degeneration caused by serum withdrawal or glutamate cytotoxicity.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.