Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation.
The protein encoded by this gene is one of the PcG proteins.
It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2).
Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior posterior axis, as well as in cell proliferation in early development.
This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin- conjugating enzyme, and possess ubiquitin ligase activity.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human RNF2. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human RNF2. Species sequence homology: Porcine (100%), Zebrafish (90%), Bovine (100%), Equine (100%), Sheep (100%), Rabbit (100%), Canine (100%), Chicken (100%), Xenopus (100%)
