84-5291-49　RFX3 100ul　527719

特徴

• Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development.
• Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis.
• Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells.
• Also required for ciliated ependymal cell differentiation.
• Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity).
• Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells.
• Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes (PubMed:20148032).
• Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells (PubMed:12411430).
• Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.