84-5291-19　RFA2 100ul　527704

特徴

• As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress.
• It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism.
• Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.
• In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation.
• Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response.
• It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage.
• Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair.
• Plays also a role in base excision repair (BER) probably through interaction with UNG.
• Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage.
• May also play a role in telomere maintenance.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.