RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation.
Modulates alternative 5'-splice site and exon selection.
Acts as a muscle cell differentiation-promoting factor.
Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation.
Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin.
Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs.
Required for the translational activation of PER1 mRNA in response to circadian clock.
Binds directly to the 3'-UTR of the PER1 mRNA.
Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation.
Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress.
Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions.
Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs.
Associates with miRNAs during muscle cell differentiation.
Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human RBM4. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human RBM4. Species sequence homology: Porcine (91%), Bovine (82%), Equine (91%), Sheep (82%), Canine (91%)
