84-5289-69　RB1CC1 100ul　527627

特徴

• Involved in autophagy (PubMed:21775823).
• Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1 (PubMed:23392225).
• Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy (By similarity).
• Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis (By similarity).
• Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways (PubMed:10769033, PubMed:12221124).
• Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111 (By similarity).
• Functions as a DNA-binding transcription factor (PubMed:12095676).
• Is a potent regulator of the RB1 pathway through induction of RB1 expression (PubMed:14533007).
• Plays a crucial role in muscular differentiation (PubMed:12163359).
• Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis (By similarity).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.