Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein.
Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction.
In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex.
Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1.
Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner.
Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Phospho-RanBP3 (Ser126/58). Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthesized peptide derived from human RanBP3 around the phosphorylation site of Ser126/58. Species sequence homology: Porcine (100%), Bovine (100%), Equine (100%), Sheep (100%), Rabbit (83%), Canine (100%), Chicken(100%)
