84-5288-01　Rac1/2/3/CDC42 200ul　527538

特徴

• Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states.
• In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
• Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages.
• Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.
• Stimulates PKN2 kinase activity.
• In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts.
• In glioma cells, promotes cell migration and invasion.
• In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning.
• Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.
• In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.
• Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.