84-5286-16 PVALB 100ul 527443
特徴
- Parvalbumin is a calcium-binding albumin protein with low molecular weight (typically 9-11kD).
- It has three EF hand motifs and is structurally related to calmodulin and troponin C.
- Parvalbumin is localised in fast-contracting muscles, where its levels are highest, and in the brain and some endocrine tissues.
- Parvalbumin is a small, stable protein containing EF-hand type calcium binding sites.
- It is involved in calcium signaling.
- Typically, this protein is broken into three domains, domains AB, CD and EF, each individually containing a helix-loop-helix motif.
- Applications:Suitable for use in Western Blot and ELISA.
- Other applications not tested.
- Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
- ELISA(peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
- Storage and Stability:May be stored at 4°C for short-term only.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Aliquots are stable for 12 months after receipt.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
- Size:100ul
- Host:rabbit
- Grade:Affinity Purified
- Purity:Purified by immunoaffinity chromatography from serum.
- Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Specificity:Recognizes endogenous levels of total PVALB. Species Crossreactivity: Mouse and rat
- Isotype:IgG
- Calc Applications Abbrev:E WB
- Calc Crossreactivity:Hu Mo Rt
- Immunogen:Fusion protein corresponding to human PVALB.
- Swiss Prot Number:P20472
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