84-5283-49　PRNP 100ul　527299

特徴

• The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures.
• The encoded protein contains a highly unstable region of five tandem octapeptide repeats.
• This gene is found on chromosome 20, approximately 20kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene.
• Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann- Straussler disease, Huntington disease-like 1, and kuru.
• An overlapping open reading frame has been found for thisgene that encodes a smaller, structurally unrelated protein, AltPrp.
• Alternative splicing results in multiple transcript variants.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.