84-5282-80　Prestin 100ul　527262

特徴

• Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ.
• Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates.
• It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate.
• After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage.
• They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization.
• As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane.
• The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state).
• So, it acts as an incomplete transporter.
• It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space.
• Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site (By similarity).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.