84-5282-33　PPP3CA 200ul　527234

特徴

• In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis.
• A group of proteins that are intimately involved in this process are the protein phosphatases.
• In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit.
• Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B and PP2C.
• An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family.
• The PP2B family comprises subfamily members PP2B-A , PP2B-A∫ and PP2B-A .
• Two additional regulatory subunits been identified, designated PP2B-B1 and PP2B-B2.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.