Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells.
Four isoforms of PP1 have been characterized: PP1 alpha, PP1 delta, PP1 gamma1 and PP1gamma2 (1).
Involvement in cell cycle regulation is one of the biological functions of PP1 (1).
It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3).
A cell cycle-dependent phosphorylation at Thr320 of PP1 alpha by cdc2 kinase inhibits PP1 alpha activity (4).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human PPP1CA. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human PPP1CA. Species sequence homology: Bovine (100%), Equine (100%), Sheep (100%), Rabbit (100%), Canine (100%)
