Transcriptional coactivator that specifically associates with either OCT1 or OCT2.
It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2.
It has no intrinsic DNA-binding activity.
It recognizes the POU domains of OCT1 and OCT2.
It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-3000Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography from serum.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of total human POU2AF1. Species Crossreactivity: mouse and rat
Isotype:IgG
Calc Applications Abbrev:E IC IF WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide corresponding to human POU2AF1 at C-terminal. Species sequence homology: porcine (90%), bovine (100%), equine (100%), sheep (100%), rabbit (91%), canine (100%), chicken (80%)