Transcriptional coactivator that specifically associates with either OCT1 or OCT2.
It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2.
It has no intrinsic DNA-binding activity.
It recognizes the POU domains of OCT1 and OCT2.
It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary Ab in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human POU2AF1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human POU2AF1 at C-terminal. Species sequence homology: porcine (%), bovine (%), equine (%), sheep (%), rabbit (%), canine (%), chicken (%)
