84-5280-18　PNPase 100ul　527120

特徴

• RNA-binding protein implicated in numerous RNA metabolic processes.
• Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction.
• Mitochondrial intermembrane factor with RNA-processing exoribonulease activity.
• Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner.
• Required for correct processing and polyadenylation of mitochondrial mRNAs.
• Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix.
• Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels.
• In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells.
• Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially.
• Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs.
• Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.