Serine/threonine-protein kinase that plays a central role in centriole duplication.
Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin.
When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase.
Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6.
Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors.
Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles.
Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1.
Phosphorylates CDC25C and CHEK2.
Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification (PubMed:22020124).
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human PLK4. Species Crossreactivity: mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to human PLK4 at N-terminal. Species sequence homology: zebrafish(90%), bovine(91%), equine(100%), sheep(91%), rabbit(100%), canine(100%), chicken(91%), xenopus (80%)
