The human urokinase-type plasminogen activator receptor (uPAR) is a 55-65kD, highly glycosylated, GPI-anchored cell surface receptor (the deglycosylated protein is 35kD) (1-3).
It is a central player in the plasminogen activation pathway.
uPAR binds with high affinity to a serine protease urokinase-type plasminogen activator (uPA) and converts plasminogen to its active form plasmin in a spatially restricted manner on the cell surface (4).
Plasmin further carries out the activation of uPA, which is inhibited by serpins, such as plasminogen activator inhibitors (5).
Therefore, uPAR plays a key role in regulating extracellular proteolysis.
In addition, uPAR plays an important role in regulating cell proliferation, adhesion, and mobility (6,7).
Research studies have shown that overexpression of uPAR is found in various cancer cells and tissues (8,9).
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human PLAU. Species Crossreactivity: Mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to human PLAU. Species sequence homology: Equine (82%), Rabbit (82%)
