84-5277-65　PIWIL2 100ul　526994

特徴

• PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells.
• Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity.
• Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells.
• Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females.
• Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8.
• Inhibits tumor cell growth when repressed.
• When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:20-1:50ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.