Protein interacting with C kinase 1 (PICK1) is a PDZ-domain containing protein that is located in the perinuclear region and is phosphorylated in response to PKC activation.
PKC , which is essential for the regulation of proliferation and differentiation in numerous cell types, contains within its catalytic region a PDZ-binding domain that is absent from other PKC isoforms.
Mutation of the PICK1 PDZ domain inhibits the binding of PICK1 to PKC .
PICK1 also interacts with the C-terminus of a-amino-3-hydroxy-5-methyl- isoxazole-4-propionic acid (AMPA) receptor, a neurotransmitter receptor located at excitatory synapses, suggesting that PICK1 plays a role in the modulation of synaptic transmission by targeting and anchoring AMPA to specific synapses.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total PICK1. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human PICK1. Species sequence homology: Porcine (100%), Bovine (93%), Equine (100%), Sheep (100%), Rabbit (87%), Canine (100%), Chicken (100%), Xenopus (87%)
