May function as a scaffold on which the coordinated assembly of proteins can occur.
May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues.
Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g.
embryonic flat bones mandible and cranium), and endochondral bone formation (e.g.
embryonic long bone development).
Plays a role during fracture repair.
Involved in BMP6 signaling pathway (By similarity).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human PDLIM7. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E FC IC IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human PDLIM7. Species sequence homology: Porcine (100%), Zebrafish (92%), Bovine (100%), Equine (100%), Sheep (100%), Rabbit (100%), Chicken (92%)
