Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor alpha (PDGFR alpha) and PDGF receptor Beta(PDGFR beta).
PDGFR alpha and PDGFR beta share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and C-terminal tail regions display a lower level (27% to 28%) of homology (1).
PDGFR alpha homodimers bind all PDGF isoforms except those containing PDGF D.
PDGFR beta homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer.
The heteromeric PDGF receptor alpha/beta binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2).
PDGFR alpha and PDGFR beta can each form heterodimers with EGFR, which is also activated by PDGF (3).
Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4).
Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLC gamma, and NCK.
A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5).
Tyr751 in the kinase-insert region of PDGFR beta is the docking site for PI3 kinase (6).
Phosphorylated pentapeptides derived from Tyr751 of PDGFR beta (pTyr751-Val- Pro-Met-Leu) inhibit the association of the C-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFR beta (7).
Tyr740 is also required for PDGFR beta-mediated PI3 kinase activation (8).
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human PDGFRA. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human PDGFRA.
