84-5273-95　PDGFB 100ul　526801

特徴

• PDGF-BB is a cystine-linked homodimer PDGF family member with key roles in development, cell proliferation, cell survival, and angiogenesis.
• PDGF-BB is expressed by vascular endothelium, megakaryocytes and Leydig cells.
• PDGF-BB targets pericytes, fibroblasts, monocytes and other cell types (1-3).
• PDGF induces fibroblast growth and migration (3) and is a chemoattractant for monocytes and granulocytes.
• Precursor PDGF-BB is cleaved intracellularly to generate a form that contains a C-terminal stretch that serves to retain PDGF-BB in the extracellular matrix.
• In a second cleavage event, the C-terminal stretch is removed extracellularly to generate mature PDGF-BB.
• PDGF-BB binds to PDGFR beta and induces receptor dimerization.
• Signaling is through the PI3K/Akt, JNK, and PLCγ pathways.
• PDGF-BB may have a role in some cancer types.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IC): 1:100-1:500ELISA (Peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.