Serine/threonine-protein kinase involved in energy homeostasis and protein translation.
Phosphorylates EEF1A1, GYS1, PDX1 and RPS6.
Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions.
Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation.
May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences.
May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency.
May also participate to respiratory regulation.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human PASK. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human PASK. Species sequence homology: Porcine (100%), zebrafish (86%), bovine (100%), equine (100%), sheep (100%), rabbit (100%), canine (100%), chicken (100%)
