Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1.
May play a role in titin/TTN downstream signaling in muscle cells.
May regulate signaling cascades through ubiquitination.
Adapter that mediates the interaction between TRAF6 and CYLD (By similarity).
May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.
Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunofluorescence (IF-IC): 1:100-1:500, Immunohistochemistry (paraffin): 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human P62/SQSTM1. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human P62/SQSTM1. Species sequence homology: Porcine (100%), Zebrafish (92%), Bovine (100%), Equine (100%), Sheep (100%), Rabbit (100%), Chicken (85%)
