84-5269-67　p21 Cip1 200ul　526578

特徴

• The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression.
• It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases.
• In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1).
• However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2).
• The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3).
• Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A is active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4).
• Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.