Stimulates GTP hydrolysis of members of the Rho family.
Its action on RHOA activity and signaling is implicated in growth and stabilization of dendritic spines, and therefore in synaptic function.
Critical for the stabilization of AMPA receptors at postsynaptic sites.
Critical for the regulation of synaptic vesicle endocytosis at presynaptic terminals.
Required for the localization of NR1D1 to dendrites, can suppress its repressor activity and protect it from proteasomal degradation (By similarity).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Oligophrenin 1. Species Crossreactivity: mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthesized peptide derived from human Oligophrenin 1. Species sequence homology: Porcine (100%), Equine (100%), Sheep (100%), Rabbit (100%), Canine (100%), Chicken (83%), Xenopus (82%)
