Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain.
Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube.
Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development (By similarity).
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography from serum.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of total human OLIG2. Species Crossreactivity: monkey
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu Mk
Immunogen:Synthetic peptide corresponding to human OLIG2. Species sequence homology: porcine (100%), canine (100%), chicken (100%), xenopus (100%)