NXF1 Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm.
The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.
Belongs to the NXF family.
Heterodimer with NXT1.
Interacts (via NTF2 domain) with NXT1.
The formation of NXF1/NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export.
Interacts with NXT2, E1B-AP5, RAE1, THOC4 and with several nucleoporins.
Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, RENT1, RENT2, RENT3A, RENT3B, THOC4 and/or REFBP2.
Found in a mRNA complex with RENT3A and RENT3B.
Interacts with FYTTD1/UIF, EIF4A3, NUPL2 and THOC5.
Interacts with Saimiriine herpesvirus 2 TIP protein
Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:3000Immunofluorescence/Immunocytochemistry (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human NXF1. Species Crossreactivity: Mouse
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human NXF1.
