Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage.
The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site.
The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination.
Primarily recognizes and repairs oxidative base damage of pyrimidines.
Has also 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity.
Acts preferentially on DNA damage opposite guanine residues in DNA.
Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted antibody in TBS, 5% milk, 0.1% Tween20 at 4°C with gentle shaking, overnight
ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human NTHL1. Species Crossreactivity: mouse and monkey
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：Synthetic peptide corresponding to human NTHL1. Species sequence homology: porcine (100%), zebrafish (100%), bovine (100%), equine (100%), chicken (100%), xenopus (100%)
