84-5260-33　NR2C2 100ul　526111

特徴

• Orphan nuclear receptor that can act as a repressor or activator of transcription.
• An important repressor of nuclear receptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways.
• May regulate gene expression during the late phase of spermatogenesis.
• Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1.
• Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences.
• Plays a fundamental role in early embryonic development and embryonic stem cells.
• Required for normal spermatogenesis and cerebellum development.
• Appears to be important for neurodevelopmentally regulated behavior (By similarity).
• Activates transcriptional activity of LHCG.
• Antagonist of PPARA-mediated transactivation.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:3000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.