84-5259-97　NPY 200ul　526093

特徴

• The NPY hormone family consists of NPY, PP and peptide YY.
• NPY (neuropeptide Y) is a 36aa protein that consists of a polyproline stretch followed by an amphipathic a-helix.
• NPY shares a 50%aa homology with pancreatic polypeptide gene (PP).
• NPY is expressed throughout the central and peripheral nervous system, particularly in the deep layers of the cortex and smaller cell bodies in the white matter.
• NPY interacts with the Y-receptor family of G protein coupled receptors.
• NPY interacts with NPY receptor Y1 to increase corticotropin levels and decrease noradrenaline levels in the hypothalamus.
• Through interactions in the hypothalus, NPY plays important roles in the regulation of energy balance by stimulating food intake.
• NPY favors energy storage by increasing lipoprotein lipase activity in white adipose tissue.
• A Leucine 7 to Proline 7 polymorphism has been implicated in an increase in alcohol preference, and decrease in cholesterol metabolism.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.