84-5259-50　NOTCH3 100ul　526069

特徴

• Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1).
• Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2).
• Binding of Notch receptors to ligands of the Delta- Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4).
• Notch3 is a member of the Notch family and is processed similar to Notch1 (5).
• It is expressed primarily in arterial smooth muscle cells (SMC).
• Mutations altering the number of cysteine residues in the Notch3 extracellular region are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a hereditary angiopathy leading to strokes and dementia in adults (6-8).
• Recent studies indicate that Notch3 is overexpressed in many types of cancer (9-11).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:200-1:1000 Immunohistochemistry: 1:25-1:100ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.