84-5258-45　NLK 100ul　526016

特徴

• The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively.
• ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif.
• Phosphorylation at both Thr-183 and Tyr-185 is required for full enzymatic activation.
• In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (5,6).
• Nlk, or nemo-like kinase, is a murine homolog of the Drosophila nemo (nmo) gene.
• Nlk and Nmo have sequence homology to both the ERK MAP kinases and the cyclin dependent kinases.
• Nlk is a nuclear protein with the ability to autophosphorylate.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.