Transcription factors of the nuclear factor kappa B (NF-kappa B)/Rel family play a pivotal role in inflammatory and immune responses (1,2).
There are five family members in mammals: RelA, c-Rel, RelB, NF- kappa B1 (p105/p50), and NF-kappa B2 (p100/p52).
Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively.
Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription.
In unstimulated cells, NF-kappa B is sequestered in the cytoplasm by I kappa B inhibitory proteins (3-5).
NF-kappa B-activating agents can induce the phosphorylation of I kappa B proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-kappa B to enter the nucleus where it regulates gene expression (6-8).
NIK and IKK alpha (IKK1) regulate the phosphorylation and processing of NF-kappa B2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
NF-kappa B assembly with I kappa B, as well as its DNA binding and transcriptional activity, are regulated by p300/CBP acetytransferases that principally target Lys218, Lys221 and Lys310 (12-14).
This process is reciprocally regulated by histone deacetylases (HDACs); several HDAC inhibitors have been shown to activate NF-kappa B (12-14).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunohistochemistry: 1:50-1:200Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human NF-kappa B p65. Species Crossreactivity: Mouse, rat and fish
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human NF-kappa B p65.
