Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation.
Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2.
Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues.
Important for G1/S transition and S phase progression.
Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.
Applications:Suitable for use in Western Blot, Immunofluorescence/Immunocytochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunofluorescence (IF-IC): 1:100-1:500ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of total human Nek9. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human Nek9. Species sequence homology: porcine (100%), bovine (100%), equine (100%), sheep (100%), rabbit (100%), canine (100%), chicken (100%)
