84-5256-13　NEFL 100ul　525894

特徴

• The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules.
• Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1).
• Similar in structure to other intermediate filament proteins, neurofilaments have a globular N-terminal head, a central alpha-helical rod domain, and a C-terminal tail.
• A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10nm intermediate filament (2).
• While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation.
• PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4).
• Neurofilament accumulations are found in many human neurological disorders including Parkinson's disease (in Lewy bodies along with alpha-synuclein), Alzheimer's disease, Charcot-Marie- Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.