84-5254-89　NCOA1 100ul　525828

特徴

• Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion.
• Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) andprostanoids (PPARs).
• Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors.
• Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear.
• Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, andpossibly acts by participating in both chromatin remodeling and recruitment of general transcription factors.
• Required with NCOA2 to control energy balance between white and brown adipose tissues.
• Required for mediating steroid hormone response.
• Isoform 2 has a higher thyroid hormonedependent transactivation activity than isoform 1 andisoform 3.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary Ab in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.