84-5254-79　NCF4 100ul　525822

特徴

• The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH- oxidase, a multicomponent enzyme system important for host defense.
• This protein is preferentially expressed in cells of myeloid lineage.
• It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation.
• This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system.
• The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events.
• The phosphorylation of this protein was found to negatively regulate the enzyme activity.
• Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Immunohistochemistry: 1:50-1:200ELISA (peptide): 1:20,000-1:40,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.